Table 2. STI identification of swab samples by qPCR and nanopore sequencing.
| Sample ID | NG | CT | TV | MG | Ct value | Read counts | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Analysed | NG | CT | TV | MG | ||||||
| 60 | - | - | - | + | 32.505 | 784 | 1 | 0 | 1 | 91 |
| 166 | - | - | - | + | 30.436 | 25188 | 2 | 0 | 9 | 19968 |
| 85 | - | - | - | + | 35.403 | 1931 | 1 | 3 | 4 | 477 |
| 31 | - | - | - | + | 34.976 | 7988 | 0 | 2 | 4 | 1416 |
| 230 | - | - | - | + | 28.800 | 23263 | 0 | 2 | 5 | 18676 |
| 103 | - | - | - | + | 33.375 | 18383 | 3 | 0 | 5 | 7262 |
| 13 | - | - | - | + | 32.980 | 26039 | 0 | 0 | 9 | 20902 |
| 109 | - | - | - | + | 29.870 | 39248 | 2 | 0 | 7 | 39248 |
| 239 | - | - | - | + | 36.210 | 3133 | 1 | 0 | 5 | 250 |
| 69 | - | - | - | + | 35.810 | 3412 | 0 | 0 | 9 | 737 |
| 263 | - | - | - | + | 29.592 | 2446 | 1 | 0 | 2 | 1457 |
| 260a | - | + | 36.672 | 633 | 1 | 1 | 4 | 1 | ||
| 51 | - | - | - | - | 2952 | 2 | 1 | 1 | 4 | |
| 75 | - | - | - | - | 589 | 0 | 2 | 4 | 2 | |
| 77 | - | - | - | - | 252 | 2 | 0 | 0 | 1 | |
| 24 | - | - | + | - | 20.607 | 20815 | 3 | 0 | 10368 | 2 |
| 58 | - | - | + | - | 25.250 | 23714 | 3 | 0 | 17712 | 2 |
| 235 | - | - | + | - | 36.170 | 29942 | 1 | 3 | 5 | 4 |
| 256 | - | - | + | - | 39.995 | 25061 | 2 | 1 | 8 | 2 |
| 146 | - | - | + | - | 39.695 | 68032 | 3 | 0 | 8 | 4 |
| 144 | - | - | + | - | 24.409 | 22359 | 1 | 2 | 6026 | 0 |
| 145 | - | - | + | - | 38.037 | 32349 | 2 | 0 | 10 | 4 |
| 261 | - | - | + | - | 38.596 | 37283 | 2 | 0 | 8 | 6 |
| 90 | - | - | + | - | 23.581 | 17230 | 2 | 1 | 9824 | 4 |
| 106 | - | - | + | - | 39.839 | 18291 | 0 | 2 | 8 | 2 |
| 92 | - | - | + | - | 40.00 | 19009 | 1 | 0 | 1 | 0 |
| 72 | - | - | + | - | 36.897 | 20163 | 2 | 0 | 11 | 2 |
| 140 | - | - | + | - | 35.249 | 22397 | 1 | 0 | 6 | 6 |
| 253 | - | - | + | - | 37.778 | 30217 | 1 | 0 | 5 | 5 |
| 41 | - | - | + | - | 38.867 | 10297 | 2 | 2 | 7 | 11 |
| 124 | - | - | + | - | 27.540 | 23714 | 2 | 1 | 17882 | 7 |
| 71 | - | - | + | - | 27.565 | 4949 | 2 | 1 | 2101 | 5 |
| 258 | - | - | + | - | 38.636 | 14925 | 10 | 17 | 5 | |
| 105 | - | - | + | - | 30.327 | 9029 | 4 | 2 | 1937 | 8 |
| 221b | - | + | - | 38.171 | 2513 | 1 | 1 | 6 | 2 | |
| 89b | - | + | - | 35.079 | 30401 | 2 | 0 | 7 | 5 | |
| 91c | - | + | - | 21.186 | 29511 | 1 | 1 | 16907 | 2 | |
| 39d | + | - | 21.381 | 41735 | 1 | 0 | 41486 | 0 | ||
| 260a | - | + | 27.556 | 35209 | 0 | 1 | 15706 | 0 | ||
| 79 | - | - | - | - | 28262 | 1 | 0 | 12 | 1 | |
| 84 | - | - | - | - | 6617 | 3 | 0 | 8 | 4 | |
| 98 | - | - | - | - | 21575 | 3 | 0 | 7 | 4 | |
| 260a | - | + | 35.905 | 17328 | 5 | 82 | 3 | 6 | ||
| 94 | - | + | - | - | 29.812 | 12745 | 3 | 530 | 4 | 3 |
| 259 | - | + | - | - | 30.331 | 8309 | 2 | 672 | 8 | 6 |
| 242 | - | + | - | - | 31.946 | 8443 | 1 | 1840 | 10 | 3 |
| 236 | - | + | - | - | 35.936 | 10335 | 3 | 65 | 7 | 8 |
| 157 | - | + | - | - | 30.126 | 10277 | 1 | 25 | 9 | 4 |
| 114 | - | + | - | - | 30.464 | 5086 | 1 | 2317 | 6 | 1 |
| 6 | - | + | - | - | 31.232 | 7582 | 1 | 127 | 18 | 24 |
| 221b | - | + | - | 35.968 | 2978 | 2 | 72 | 4 | 3 | |
| 89b | - | + | - | 29.932 | 6565 | 0 | 220 | 10 | 3 | |
| 30e | + | - | - | 30.886 | 14878 | 2 | 13796 | 3 | 1 | |
| 39d | + | - | 30.536 | 999 | 1 | 797 | 3 | 1 | ||
| 102 | - | - | - | - | 3095 | 0 | 7 | 5 | 1 | |
| 107 | - | - | - | - | 4664 | 2 | 1 | 7 | 2 | |
| 125 | - | - | - | - | 101 | 1 | 0 | 9 | 2 | |
| 30e | + | - | - | 19.033 | 27210 | 21087 | 0 | 1 | 0 | |
| 91c | + | - | - | 20.035 | 39223 | 36590 | 1 | 5 | 0 | |
| 39d | + | - | 21.504 | 28317 | 12325 | 3 | 3 | 0 | ||
| 126 | - | - | - | - | 14121 | 2 | 0 | 2 | 1 | |
| 189 | - | - | - | - | 9168 | 3 | 1 | 1 | 5 | |
| 193 | - | - | - | - | 11334 | 1 | 0 | 3 | 2 | |
| NG positive control | 7937 | 7659 | 0 | 1 | 0 | |||||
a CT, TV and MG mixed infection.
b CT and TV mixed infection.
c NG and TV mixed infection.
d NG, CT and TV mixed infection.
e Ng and CT mixed infection.