Table 3. BLAST confirmation of antimicrobial resistance associated mutations identified by single gene targeted PCR nanopore sequencing*.
| Bacterial gene | Sample | Antimicrobial resistance region: nucleotide sequence (NS) and amino acid (AA) change |
|---|---|---|
| Neisseria gonorrhoeae: gyrA | Wild type | NS: -TCCGCAGTTTACGAC- |
| AA: S91 D95 | ||
| All NG samples | NS -TTCGCAGTTTACGCC- | |
| AA change: S91F D95A | ||
| Positive control | NS: -TCCGCAGTTTACGAC- | |
| AA: S91 D95 | ||
| Mycoplasma genitalium: 23S rRNA gene | Wild type | NS: -CGGGACGGAAAGACC- |
| A2058, A2059 (Escherichia coli numbering) | ||
| All MG samples | NS: -CGGGACGGAAAGACC- | |
| A2058, A2059 (Escherichia coli numbering) | ||
| Trichomonas vaginalis: ntr6 | Wild type | NS: -AATGCAAAAGCAGAC- |
| AA: K80 | ||
| 2 TV samples | NS: -AATGCAATAGCAGAC- | |
| AA change: K80STOP | ||
| 8 TV samples | NS: -AATGCAAAAGCAGAC- | |
| AA: K80 |
*One or more changes of the bold bases in the wild type cause antimicrobial resistance of strains.