Figure 3.

Memory impairment induced by HFHSD exposure. Number of arm entries in the Y-maze and spontaneous alternation (A) were not modified during exposure to HFHSD. After familiarization with 2 objects, both novel location recognition (B) and novel object recognition (C) tasks show that mice exposed to HFHSD for 24 weeks did not display increased exploration of novel location or object. When diet was reversed, mice recovered the ability to recognize novelty in the object displacement task (D) but not in the object replacement task (E). Dashed lines in graphs represent chance (50%). Data is mean±SD. Letters over data-points indicate significant differences relative to CD or as indicated (^a P<0.05, ^b P<0.01, ^c P<0.001) based on Fisher’s LSD post hoc comparison following presence of significant effects of diet or interaction between diet and time in ANOVA tests.