| PBMC sampling timepoint |
Baseline |
1 month post-third vaccination |
| PBMC stimulation |
Vehicle (DMSO); stimulated on site before cryopreservation of cell pellets for subsequent RNA extraction. |
24-hour antigen (CSP) stimulation adjusted by vehicle (DMSO); PBMC were cryopreserved before stimulation |
| Background correction |
N/A |
Yes, subtraction of expression in vehicle-stimulated PBMC (thus the observed response is specific to antigen stimulation, i.e. recall response) |
| Presence of monocyte-related BTMs |
Yes, of the 45 BTMs, 7 were monocyte-related: M81, M118.1, M11.0, M118.0, S4, M73, M4.15 |
Yes, of the 24 BTMs, 3 were monocyte-related: M81, M118.1, M11.0 |
| Gene expression measurement |
RNA-seq |
Microarray |
| Hypothesis: |
Stabilin-1high immunosuppressive monocytes circulating at baseline may inhibit protective RTS,S-induced T-cell responses [supported by (18)] or another RTS,S-induced protective adaptive response. |
Protected individuals may have monocytes that are qualitatively superior in mediating, e.g., Fc receptor/antibody-dependent responses (reflected by an altered transcriptional profile), resulting in improved control of infection. |
