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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Methods Mol Biol. 2019;2006:341–353. doi: 10.1007/978-1-4939-9566-0_23

Table 3:

Preparation of Tyrodes’s Albumin1, Lactate, and Pyruvate1 (TALP) Medium.

Component Amount per 500 mL Concentration (mM)
Sodium Chloride Stock 300 mL 114.0
Phenol Red 0.005 g 28.2
Sodium D,L-Lactate Stock 33.5 mL 10.0
Gentamicin Sulfate 25 mg 0.2
Potassium Chloride Stock 9.5 mL 3.2
Calcium Chloride Dihydrate Stock 8.5 mL 2.0
Magnesium Chloride Hexahydrate Stock 2.05 mL 0.5
Sodium Bicarbonate 75 mL 25.0
Sodium Phosphate Stock2 8.5 mL 0.3
1

Add 3 mg/mL BSA and 0.006g/100mL (0.5 mM) sodium pyruvate prior to use

2

Prepared in glucose stock solution for a final concentration of 5.0 mM glucose.