Table 1.
Primers used for RT-qPCR analysis of lung gene expression
| Gene | Name | Forward Primer | Reverse Primer |
|---|---|---|---|
| Acta2 | Actin alpha 2 | TGTGCTGGACTCTGGAGATG | GAAGGAATAGCCACGCTCAG |
| Actg | Actin gamma | GGATCGGTGGCTCCATTCTG | TGAGGTGTGTACATTTGCCAG |
| Adipoq | Adiponectin | TGTTCCTCTTAATCCTGCCCA | CCAACCTGCACAAGTTCCCTT |
| Ccl2 | Chemokine (C-C motif) ligand 2 | TCCCTGTCATGCTTCTGGGC | GTTCTGATCTCATTTGGTTCCGATCC |
| Clca1 | Chloride channel accessory 1 | AAGCAAACCACTCCCATGAC | TGCGAAAGCATCAACAAGAC |
| Col1a1 | Collagen type I alpha I | GAGCGGAGAGTACTGGATCG | GTTCGGGCTGATGTACCAGT |
| Il1b | Interleukin 1 beta | GCCCATCCTCTGTGACTCAT | AGGCCACAGGTATTTTGTCG |
| Il6 | Interleukin 6 | CCGGAGAGGAGACTTCACAG | GAGCATTGGAAATTGGGGTA |
| Il17a | Interleukin 17 A | CTGCTGAGCCTGGCGGCTAC | GGCGGCACTGAGCTTCCCAG |
| Muc5ac | Mucin 5 subtype AC | CCATGCAGAGTCCTCAGAACAA | TTACTGGAAAGGCCCAAGCA |
| Muc5b | Mucin 5 subtype B | ACACATGCACCTGCCTCTCTGA | TCCATGGAGTACTTGGATATTC |
| Myh11 | Myosin heavy polypeptide 11 | CTCTGGCCTCTTCTGTGTGG | TCTTTCTTGCCCTTGTGGGA |
| Myod | Myogenic differentiation 1 | GCCGCCTGAGCAAAGTGAATG | CAGCGGTCCAGGTGCGTAGAAG |
| Myog | Myogenin | CTACAGGCCTTGCTCAGCTC | ACGATGGACGTAAGGGAGTG |
| Nlrp3 | NRL family pyrin domain containing 3 | ATGCTGCTTCGACATCTCCT | AACCAATGCGAGATCCTGAC |
| Rpl13a | Ribosomal protein L13a | CCCTCCACCCTATGACAAGA | CTGCCTGTTTCCGTAACCTC |
| Saa3 | Serum amyloid A3 | CAGGATGAAGCCTTCCATTG | CATGACTGGGAACAACAGGA |
| Tagln | Transgelin | GCGGCCTTTAAACCCCTCA | CTCCACTAGTCGCTCCTCCA |
| Tjp1 | Tight junction protein 1 | CCACCTCTGTCCAGCTCTTC | CACCGGAGTGATGGTTTTCT |
| Tnfa | Tumor necrosis factor | TCCCAGGTTCTCTTCAAGGGA | GGTGAGGAGCACGTAGTCGC |
| Vim | Vimentin | TGCTTCAAGACTCGGTGGAC | AAGCGCACCTTGTCGATGTA |