Figure 5.

Ablation of interleukin-1 receptor type 1 (IL-1R1) diminishes Akt phosphorylation in podocytes after nephrotoxic serum (NTS). A: Western blot assay showing Akt phosphorylation (Ser⁴⁷³) in glomeruli isolated from wild-type (WT) and podocyte-specific knockout (PKO) mice in the different groups. B: semiquantitative determination of Akt phosphorylation from the blots shown in A (n = 3–8). C: representative immunostaining for Akt phosphorylation in glomeruli from the different groups. P values in B were analyzed by one-way ANOVA followed by a Student–Newman–Keuls test. *P < 0.05. Scale bar = 40 µm. p-Akt, phosphorylated Akt; Veh, vehicle.