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. 2021 Nov 16;41(4):538–549. doi: 10.1038/s41388-021-02114-8

Fig. 2. CBX3 promotes LUAD cell growth and invasion.

Fig. 2

a Analysis the potential cancer-related function of CBX3 in lung adenocarcinoma by the single cell sequencing dataset. b–g A549 and H1299 cells were infected with shControl, shCBX3 #1, or shCBX3 #2. Seventy two hours post infection, cells were collected for Western blotting analysis (b), RT-qPCR analysis (c), MTS assay (d), colony formation assay (e and f) and transwell assay (g). Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data presented as Mean ± SD with three replicates (n = 3). ***P < 0.001. h–k A549 and H1299 were transfected with pcDNA3.1 (as empty vector) or Flag-CBX3 for 24 h. Cells were collected for RT-qPCR analysis (h), MTS assay (i) and transwell assay (j, k). Statistical significance was determined by two-side student t test. Data presented as Mean ± SD with three replicates (n = 3). ***P < 0.001. l, m A549 cells were infected with Tsin-EV + shControl, Tsin-EV + shCBX3, or Tsin-CBX3 + shCBX3 for 72 h. Cells were harvested for Western blotting analysis (l) and MTS assay (m). Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data presented as Mean ± SD with three replicates (n = 3). ***P < 0.001. n–p A549 cells were infected with Tsin-EV + shControl, Tsin-EV + shCBX3, or Tsin-CBX3 + shCBX3 as indicated. After 72 h puromycine selection, cells were harvested and subcutaneously injected into nude mice for xenografts assay. The image of tumor was shown in panel (n). The tumor growth curve was indicated in panel (o). The tumor mass was demonstrated in panel (p). Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data presented as Mean ± SD with five replicates. NS not significant; ***P < 0.001.