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. 2021 Nov 16;41(4):571–585. doi: 10.1038/s41388-021-02113-9

Fig. 4. NAA40-mediated regulation of one-carbon metabolism, histone methylation and cell viability are dependent on its acetyltransferase activity.

Fig. 4

A Western blot analysis (N = 3) of HCT116 inducible NAA40-KD cells transduced with Empty vector, shRNA-Resistant NAA40(WT)-V5 or shRNA-Resistant NAA40(E139Q)-V5 plasmids and treated with or without doxycycline using antibodies against the specified antibodies (left panel). Representative confocal images of V5-tag (green) or Hoechst (blue) in the indicated stable cell lines treated with or without doxycycline (right panel). Scale bar, 100 μm. B MTT assay (mean ± s.d., N = 3) to assess cell viability in dox-treated NAA40-KD2 cells expressing the indicated proteins. C qRT-PCR analysis (mean ± s.d., N = 3) demonstrating the mRNA levels of NAA40, TYMS, MTHFR and MAT1A normalized to β-actin in HCT116 inducible NAA40-KD cells transduced with the indicated plasmids in the presence or absence of dox treatment. All statistical analyses were performed using unpaired two-tailed Student’s t test (ns = no significance, *p < 0.05, **p < 0.01, ****p < 0.0001).