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. 2022 Jan 21;12:1149. doi: 10.1038/s41598-022-05136-8

Table 2.

Percentages of extended primers with NTPs at the first position next to the 3′ terminal of each primer in 0 or 20 wt% PEG200.

Substrate Primer
Primer A Primer C Primer G (Matched) Primer U
(-)PEG200 ( +)PEG200 (−)PEG200 ( +)PEG200 (−)PEG200 ( +)PEG200 (−)PEG200 ( +)PEG200
ATP 0a 0a 30 ± 2.2 39 ± 1.4 9.0 ± 2.2b 25 ± 8.1b 8.8 ± 0.6 13 ± 1.7
CTP 7.5 ± 1.2 18 ± 1.7 15 ± 1.7 30 ± 0.6 29 ± 11b 35 ± 13b 13 ± 0.9 25 ± 1.2
GTP 21 ± 7.0 28 ± 5.0 33 ± 5.2 56 ± 10 23 ± 12b 33 ± 20b 18 ± 1.1 34 ± 7.1
UTP (Matched) 8.4 ± 1.4 17 ± 3.4 12 ± 0.7 14 ± 1.5 50 ± 14b 37 ± 20b 16 ± 4.1 17 ± 4.1

aNo bands for extended primers were detectable.

bData were taken from the reference26.