Table 2.
Localization and methylation of CpG sites of 5 differentially methylated genes analyzed by targeted bisulfite massive parallel sequencing in chorionic villi of miscarriages with trisomy 16.
| Region | Strand | Genomic coordinates (hg19) | CpG | DMS | DMS/all CpG sites, % |
|---|---|---|---|---|---|
| ANKRD53_1 | + | chr2: 71206056–71206605 | 40 | 6 | 15 |
| ANKRD53 in total | 40 | 6 | 15 | ||
| CALCB_1 | + | chr11: 15094027–15094302 | 15 | 3 | 20 |
| CALCB_3 | + | chr11: 15094516–15094823 | 8 | 4 | 50 |
| CALCB_4 | + | chr11: 15094798–15095066 | 12 | 4 | 33 |
| CALCB in total | 35 | 11 | 31 | ||
| GATA3_1 | + | chr10: 8091317–8091687 | 30 | 0 | 0 |
| GATA3_2 | + | chr10: 8092469–8093016 | 57 | 0 | 0 |
| GATA3_4 | + | chr10: 8093670–8094118 | 37 | 21 | 57 |
| GATA3_5 | + | chr10: 8094186–8094771 | 32 | 23 | 72 |
| GATA3_8 | + | chr10: 8095096–8095448 | 15 | 3 | 20 |
| GATA3-AS1 in total | 84 | 47 | 56 | ||
| SLC13A4_1 | − | chr7: 135412984–135413410 | 11 | 0 | 0 |
| SLC13A4_2 | − | chr7: 135412563–135413021 | 7 | 6 | 86 |
| SCL13A4 in total | 18 | 6 | 33 | ||
| TRPV6_1 | − | chr7: 142583430–142583865 | 5 | 0 | 0 |
| TRPV6_2 | − | chr7: 142582950–142583429 | 11 | 6 | 54 |
| TRPV6_3 | − | chr7: 142582830–142583240 | 6 | 5 | 83 |
| TRPV6 in total | 22 | 11 | 50 |