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. 2022 Jan 21;13(1):71. doi: 10.1038/s41419-022-04516-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A RICH1 overexpression in 4T1 cells. B In vivo tumor formation of the mice injected with serial dilutions of 4T1-Control and 4T1-RICH1 cells. C Tumor images of mice injected with 50,000 control and RICH1-overexpressing 4T1 cells. D, E. Tumor weights (D) and volumes (E) of mice injected with 50,000 control and RICH1-overexpressing 4T1 cells. F Representative IHC staining results of tumor samples. G Representative visualized lung metastasis models and HE staining of lung metastasis specimen (arrows: metastatic foci). H Number of metastatic foci of control and RICH1-overexpressing 4T1 cells. I Lung metastasis area of control and RICH1-overexpressing 4T1 cells. Statistical significance was determined by two-tailed unpaired t-test. Abbreviation: *p < 0.05; **p < 0.01; ***p < 0.001.