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. 2021 Sep 18;19(1):161–175. doi: 10.1007/s13770-021-00391-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Characterization of MVF after enzymatic digestion of murine adipose tissue with collagenase NB 4 Standard Grade. A, B Number (A, given per mL adipose tissue) and length distribution (B, given in %) of MVF isolated from visceral (vc, gray bars, n = 3) and subcutaneous (sc, black bars, n = 3) adipose tissue of donor mice. Means ± SEM. C, D Light microscopic images of MVF isolates from visceral (C) and subcutaneous (D) adipose tissue (arrows = MVF; arrowheads = aggregates of undigested connective tissue fibers). Scale bars: 150 µm. E, F Fluorescence microscopic images of PI-stained MVF from visceral (E) and subcutaneous (F) adipose tissue of donor mice for the assessment of cell viability (arrows = dead PI⁺ cells). Cell nuclei were stained with Hoechst 33342. Scale bars: 65 μm. G PI⁺ cells (given in % of all counted cells) of MVF from visceral (vc, gray bar, n = 8) and subcutaneous (sc, black bar, n = 8) adipose tissue of donor mice. Means ± SEM