Table 2.
Diameter (µm), centerline RBC velocity (µm/s) and wall shear rate (s−1) of individual microvessels within CGAG scaffolds, which were seeded with visceral (vc; n = 8) or subcutaneous (sc; n = 8) MVF isolates from GFP+ C57BL/6 donor mice, directly (d0) as well as 3, 6, 10 and 14 days after implantation, as assessed by intravital fluorescence microscopy and computer-assisted image analysis
| Microvessels | 0d | 3d | 6d | 10d | 14d |
|---|---|---|---|---|---|
| Diameter (µm): | |||||
| vc | – | 33.2 ± 10.5 | 25.4 ± 2.9 | 21.5 ± 2.7 | 15.8 ± 1.3 |
| sc | – | 19.9 ± 8.7 | 26.2 ± 3.5 | 20.6 ± 2.4 | 20.8 ± 2.0* |
| Centerline RBC velocity (µm/s): | |||||
| vc | – | 63.8 ± 12.5 | 131.7 ± 39.1 | 151.3 ± 22.9 | 276.8 ± 20.3 |
| sc | – | 49.0 ± 1.0 | 184.2 ± 70.4 | 188.6 ± 52.6 | 128.9 ± 31.4* |
| Wall shear rate (s−1): | |||||
| vc | – | 13.0 ± 5.1 | 53.7 ± 17.4 | 69.6 ± 23.2 | 160.8 ± 12.9 |
| sc | – | 80.6 ± 31.9 | 95.1 ± 53.4 | 98.6 ± 38.1 | 64.3 ± 22.4* |
Mean ± SEM
*p < 0.05 vs. vc