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. 2022 Jan 21;13(1):73. doi: 10.1038/s41419-022-04524-2

Fig. 6. NKAP recruited SFPQ to regulate the TTS splicing event and mRNA maturation.

Fig. 6

A Schematic of Co-IP experiment and proteomic screening by MS to identify the potential mechanism. B SFPQ knockdown decreased protein levels of SLC7A11. U2AF2 knockdown had no effect on SLC7A11 expression. C Co-IP analysis of the interaction between Flag-NKAP and SFPQ, which could be blocked by treatment with either cycloleucine or METTL3 RNAi. D Chart showed ASprofile analysis and statistics of alternative splicing events for all transcripts based on high-throughput RNA sequencing. E Decreased occurrence of alternative transcription termination site (TTS) on the SLC7A11 transcript. F Sashimi plots showing retained exon on the SLC7A11 transcript in both shNKAP and siMETTL3 groups. G Schematic representation of strategy to identify intron (unspliced, pre-mRNA). H qPCR showed that the pre-mRNA/mRNA ratio of SLC7A11 increased significantly in the shNKAP group. GAPDH was used as an internal control in western blot. All bars show mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.