Fig. 6. Using transcriptional valves to regulate an array of CRISPR sgRNAs.

a Normalized dRNA-seq read depth profile for each array. Dotted lines denote the start and end of each valve. General array design is shown at the bottom, with the P_T7 promoter followed by 3 guide RNAs (gRNAs) separated by two transcriptional valves (V1, V2). Resulting gRNA stoichiometries are indicated in top left of each profile (gRNA1:gRNA2:gRNA3) and valve designs are shown above their respective parts. b Comparison of predicted (lighter shading) and measured (darker shading) termination efficiency (T_e) for each valve in the arrays. Bars are colored by valve position. c Comparison of T_e measurements for in vitro transcription reactions performed with varying concentrations of T7 RNA polymerase (RNAP). Each point corresponds to a transcriptional valve. R² is the square of the Pearson correlation coefficient. Source data are provided as a Source Data file.