Table 2.
Cell wall composition of mandarin pectin-rich polysaccharides (M-PRP) measured using semi-quantitative ELISA. Data is mean of five replicate wells at A450 nm ± standard deviation, with stars representing relative strength of absorbance.
| Class | Epitope | Antibody | Absorbance |
|---|---|---|---|
| Hemicellulose | Xyloglucan | LM25 | 0.62 ± 0.03* |
| Pectin | Un-esterified HG | LM19 | 1.70 ± 0.20*** |
| Esterified HG | LM20 | 0.78 ± 0.05* | |
| RGI galactan | LM5 | 1.88 ± 0.08*** | |
| RGI arabinan | LM6-M | 1.61 ± 0.08** | |
| RGI linear arabinan | LM13 | 0.31 ± 0.06 | |
| Protein | Extensin | JIM20 | 0.82 ± 0.12* |
HG = homogalacturonan; RGI = rhamnogalacturonan.