Figure 2.

Vector characterization in MEG-01 cells

(A) Vector titers of all three vectors. (B) Representative flow cytometry plots. (C) Transduction efficiency. (D) MFI of GFP⁺ cells normalized to mock MFI. (E) VCN and (F) GFP mRNA expression in cells transduced with MOIs 0.075, 0.13, 0.25, and 0.6 of the RSD, TDCT, or control vector. Data represent mean ± SD, n = 3–5 independent cultures; in (A) one-way ANOVA, ns, no significant difference; (C–F) two-way ANOVA, value from one vector at one MOI was compared with the other two vectors at the same MOIs; significance is relative to RSD and control; ∗p < 0.05, ∗∗p < 0.01. (G) Flow cytometric histograms showing surface GFP expression in MEG-01 cells transduced with TDCT (MOI 0.25 and 1) in comparison with RSD and untransduced cells. GFP was detected by antibody staining (clone 1A12-6-18, BD Bioscience). (H) Summarized quantification of the MFI of cell surface GFP on MEG-01 cells. n = 3 independent transductions, mean ± SD, one-way ANOVA, ∗∗∗∗p < 0.0001. MOI, multiplicity of infection; MFI, mean fluorescence intensity; VCN, vector copy number.