Figure 9.

SNHG26 directly interacts with PGK1 in TSCC cells

(A) Silver-stained SDS-PAGE gel of proteins immunoprecipitated from CAL27 cell extract by the sense and antisense RNA of SNHG26. The two lanes were used for mass spectrum determination using liquid chromatography dual mass spectrometry. The frame indicates PGK1. (B) Analysis of the interaction propensities between SNHG26 and PGK1 using catRAPID tool. (C) RNA pull-down assay conducted using biotin-labeled SNHG26 probe and PGK1 expression was determined by western blot assay. (D) Amount of SNHG26 bound to SNRNP70 (a positive control), PGK1, or IgG (a negative control) was detected by qRT-PCR after RIP in TSCC cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.