Confirmation of the distinct gene signatures of LDNs and HDNs. (A–C) Expression of representative genes in MYC target, NF-κB pathways, and surface markers of LDNs and HDNs by qRT-PCR (n = 4). Means ± SD are shown. The P value is shown under each panel. (D) Immunofluorescence staining (magnification: 200× and 1000×) of S100A8 (green), CXCR2 (pink), and TLR4 (red) in LDNs and HDNs. (E) The expression levels of S100a8 and S100a9 in LDNs and HDNs by qRT-PCR (n = 4). Means ± SD are shown. P values are shown on the panel. CXCR2, C-X-C Motif Chemokine Receptor 2; DAPI, 4′,6-diamidino-2-phenylindole.