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. 2021 Nov 13;13(3):717–737. doi: 10.1016/j.jcmgh.2021.11.003

Table 1.

Antibody Information

Antibody Company Cat# Applications and dilutions
S100A8 Novus Biologicals, Littleton, CO MAB3059 Fluorescent staining 1:300
PCNA Santa Cruz, Dallas, TX sc56 Fluorescent staining 1:100
MUC2 Invitrogen PA5-21329 Fluorescent staining 1:200
PD-L1 R&D Systems AF1019-SP Fluorescent staining 1:100
SMA Sigma SAB5500002 Fluorescent staining 1:100
CXCR2 Thermo Fisher PA5-100951 Fluorescent staining 1:100
TLR4 Novus Biologicals NB100-56566SS Fluorescent staining 1:100
IL1b Abcam, Cambridge, MA Ab9722 Fluorescent staining 1:100
p-iκBα Cell Signaling, Danvers, MA 9246 Western blot 1:1000
p-ERK Cell Signaling 9101 Western blot 1:1000
ERK Cell Signaling 9102 Western blot 1:1000
p-p38 Cell Signaling 9215 Western blot 1:1000
p38 Cell Signaling 9212 Western blot 1:1000
PKA substrate Cell Signaling 9621 Western blot 1:1000
β-actin Sigma A1978 Western blot 1:5000
CD8 BioLegend 300912 Flow cytometry 1:200
CD4 BioLegend 317427 Flow cytometry 1:200
Ly6G BD Biosciences 551460 Flow cytometry 1:200
CD11b Thermo Fisher 12-0112-82 Flow cytometry 1:200

CXCR2, C-X-C Motif Chemokine Receptor 2; MUC2, Mucin 2; p-ERK, phosphorylated ERK; p-iκBα, phosphorylated iκBα; PCNA, proliferating cell nuclear antigen; PKA, adenosine 3′,5′-cyclic monophosphate–dependent protein kinase; SMA, Smooth muscle actin.