Table 1.
Antibody | Company | Cat# | Applications and dilutions |
---|---|---|---|
S100A8 | Novus Biologicals, Littleton, CO | MAB3059 | Fluorescent staining 1:300 |
PCNA | Santa Cruz, Dallas, TX | sc56 | Fluorescent staining 1:100 |
MUC2 | Invitrogen | PA5-21329 | Fluorescent staining 1:200 |
PD-L1 | R&D Systems | AF1019-SP | Fluorescent staining 1:100 |
SMA | Sigma | SAB5500002 | Fluorescent staining 1:100 |
CXCR2 | Thermo Fisher | PA5-100951 | Fluorescent staining 1:100 |
TLR4 | Novus Biologicals | NB100-56566SS | Fluorescent staining 1:100 |
IL1b | Abcam, Cambridge, MA | Ab9722 | Fluorescent staining 1:100 |
p-iκBα | Cell Signaling, Danvers, MA | 9246 | Western blot 1:1000 |
p-ERK | Cell Signaling | 9101 | Western blot 1:1000 |
ERK | Cell Signaling | 9102 | Western blot 1:1000 |
p-p38 | Cell Signaling | 9215 | Western blot 1:1000 |
p38 | Cell Signaling | 9212 | Western blot 1:1000 |
PKA substrate | Cell Signaling | 9621 | Western blot 1:1000 |
β-actin | Sigma | A1978 | Western blot 1:5000 |
CD8 | BioLegend | 300912 | Flow cytometry 1:200 |
CD4 | BioLegend | 317427 | Flow cytometry 1:200 |
Ly6G | BD Biosciences | 551460 | Flow cytometry 1:200 |
CD11b | Thermo Fisher | 12-0112-82 | Flow cytometry 1:200 |
CXCR2, C-X-C Motif Chemokine Receptor 2; MUC2, Mucin 2; p-ERK, phosphorylated ERK; p-iκBα, phosphorylated iκBα; PCNA, proliferating cell nuclear antigen; PKA, adenosine 3′,5′-cyclic monophosphate–dependent protein kinase; SMA, Smooth muscle actin.