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Process for FRAP imaging
(A) Open CO2 tank valve and turn on a hit stage top incubation system.
(B) Combine coverslip with a quick-release imaging chamber and place it to the live chamber.
(C) Set laser intensity for photo-bleaching.
(D) Representative images before or after photo-bleaching. Scale bar, 5 μm.
(E) Setting for recording recovery of fluorescence intensity.
