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. 2022 Jan 20;3(1):101118. doi: 10.1016/j.xpro.2021.101118

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© 2021 Northwestern University

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Process for analyzing fluorescence intensity

(A) Individual settings to analyze fluorescence intensity in the region of interest of a HEK293T cell using ImageJ.

(B) FRAP curve of SEP-DAGLα in HEK293T cells.

(C) Individual settings to analyze fluorescence intensity in the region of interest of a primary cultured neuron using ImageJ.

(D) FRAP curve of SEP-DAGLα in primary cultured neurons. Normalize the intensity of FITC by TRITC. One-phase exponential equations fitted the curves. t_1/2: the half time of equilibrium; IF: immobile fraction; MF: mobile fraction.