Fig. 4.

IgG anti-RBDf response in mice assayed by ELISA. The chart shows means of titers with standard error media (n = 5). The statistical analysis was performed using Kruskal–Wallis and Dunn’s multiple comparison tests (*p < 0.05; **p < 0.01). All the statistical differences are shown and were detected only compared to the placebo group (HBc alum SC/IN). SC: subcutaneous; IN: intranasal; MIX: simple mixture. Numbers on top of the bars are the antibody titer means. The dashed line indicates the cutoff value.