FIGURE 3.

Effect of donor nephrectomy on urinary extracellular vesicle (uEV) excretion. (a) uEV excretion before (Pre) versus after (Post) donor nephrectomy measured by EVQuant in 24‐h urine; (b) Urinary excretion of CD9‐ uEVs (left panel) and CD9+ uEVs (right panel) before and after donor nephrectomy measured by EVQuant in 24‐h urine; (c) Spot uEV/creatinine before and after donor nephrectomy measured by EVQuant; (d) Spot uEV/creatinine ratios of CD9‐ (left panel) and CD9+ uEVs (right panel) before and after donor nephrectomy measured by EVQuant; (e) Spot uEV/creatinine before versus after donor nephrectomy measured by nanoparticle tracking analysis (NTA); (f) Size distribution of uEVs by NTA (left panel) and percentage change of size distribution (right panel, uEV/creatinine ratio ± SEM per 1 nm size bin; (g) CD9‐Europium signal to urine creatinine ratio (Lum./creat) before and after donor nephrectomy measured by CD9–TR‐FIA (signal pre‐donation normalized to 1); (h) Representative immunoblots of CD9 and TSG101 in the 200K uEV pellet before and after donor nephrectomy, loaded relative to individual urine creatinine concentrations, with corresponding densitometry. *p ≤ 0.05, **p < 0.01, ***p < 0.001