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. 2022 Jan 22;22:47. doi: 10.1186/s12870-022-03440-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A Transcription activity assay of SsCUC2. The ORF of SsCUC2 was introduced into the yeast expression vector pGBKT7. Yeast cells cotransformed with pGBKT7-53 + pGADT7-T were used as the positive control, and yeast cells with empty vector pGBKT7 were used as a negative control. The yeast cultures harboring respective vectors were grown on the synthetic medium supplied with dextrose (SD) in the absence of Trp (SD/ -T, left panel), on SD medium in the absence of Leu, Trp, and His (SD/ -L -T -H, middle panel) and on SD medium with α-galactosidase and in the absence of Leu, Trp, and His (SD/ -L -T –H + X-α-gal, right panel). Yeast cells were incubated until OD₆₀₀ reached 1 and then diluted 10- and 100-fold for assays. B Subcellular localization of SsCUC2 in tobacco epidermal cells (lower panel)