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. 2022 Jan 22;22:47. doi: 10.1186/s12870-022-03440-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Expression and regulation of sugarcane CUC genes. A The expression patterns of SsCUC2, SsCUC3 and miR164a in different developmental stages of ovules. The expression level for the AC stage was set to 1.0, and the data presented in MMC, Meiosis, Mitosis and Mature is relative to AC. B Predicted target site in SsCUC2 by psRNATarget database. C Target plot (T-plot) indicating the cleavage event mediated by miR164 on the PARE-seq. D RT-qPCR of SsCUC2, SsCUC3 and miR164a in different developmental stages of ovules. Sugarcane β-actin was used as an internal control. The expression level was set to 1.0 (for AC), and the data presented relative transcript abundance to AC. The threshold cycle (CT) values were used to calculate relative expression using the 2^−ΔΔCT method. The data represent the means ± SE of three replicates, and asterisks denote statistically significant values (**p < 0.01 and *** p < 0.001)