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Functional characterization of sugarcane CUC2 gene. A&B) Hygromycin-based screening of T1 transgenic plants. The plants carrying the SsCUC2 gene show normal two separate cotyledons (marked with white arrows), whereas the non-transformed with the cup-shaped fused cotyledon. Two complemented T2 lines (Com1 and Com2) showing the rescue of mutant phenotype C) at the early developmental stage with two separate cotyledons D) at the reproductive stage with normal inflorescences
