Figure 1.

The workflow of specimen preparation and imaging, along with examples of different imaging modalities and a sample 3D printed mould. The six images show a coronal section from the 0.4 × 0.4 × 0.4 mm³ 7 T MRI, which is used to generate 3D printed moulds and to correct 9.4 T scans for distortion; a coronal section from the 0.2 × 0.2 × 0.2 mm³ 9.4 T MRI; intact MTL specimen placed in the cutting mould 3D printed from the 7 T MRI; a block-face photograph taken during cryosectioning; a Nissl-stained histology slide; and an anti-tau IHC slide counterstained for Nissl.