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. 2021 Dec 22;6(24):e146362. doi: 10.1172/jci.insight.146362

Figure 6. Rescue experiments verify that CPVL regulates apoptosis of glioma cells via the IFN-γ/STAT1 signaling pathway.

Figure 6

(A) MTT assays were used to investigate the cell proliferation rates of CPVL-silenced U251 cells treated with fludarabine compared with the control cells (n = 3). (B) Colony formation assay was used to investigate the cell proliferation capacity of CPVL-silenced U251 cells treated with fludarabine, compared with the control cells. Representative pictures are shown on the left, and the number of colonies counted is shown on the right (n = 3). (C) FACS assay was used to detect the effect of fludarabine treatment on cell apoptosis in CPVL-silenced U251 cells, compared with the control cells (n = 3). Representative profiles are shown on the left, and the percentages of cells that were statistically analyzed are shown on the right. (D) Cell cycle assays were used to investigate the influence of fludarabine treatment on the CPVL-silenced U251 cell cycle compared with the control cells (n = 3). The fractions of viable cells in the G1, S, and G2-M phases were quantified by flow cytometry. Representative profiles were shown on the left, and the percentages of cells that were statistically analyzed are shown on the right. All experiments were conducted in triplicate. Bar graph data are presented as the mean ± SD. Two-tailed Student’s t test analyses were performed.*P < 0.05.