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. 2021 Dec 22;6(24):e154093. doi: 10.1172/jci.insight.154093

Figure 3. Flow cytometry of NHP fetal liver immune cells.

Figure 3

Nonlymphoid myeloid cells and lymphoid cells in CD (blue) and WSD (yellow) fetal liver mononuclear cells, as a proportion of total viable cells or cell subsets by flow cytometry; n = 4 CD and n = 7 WSD. (A) Nonlymphoid cells, defined as CD3CD20, as a proportion of total viable cells. (B) DCs (DC), myeloid DCs (mDC), and plasmacytoid DCs (pDC) as a proportion of total viable cells. (C) Monocytes and natural killer (NK) cells as a proportion of nonlymphoid cells. NK cell subsets as a proportion of NK cells (D) and monocyte subsets as a proportion of monocytes (E) characterized by presence (16+) or absence (16-) of CD16. (F) CD4+ T cells, CD8+ T cells, and CD20+ B cells as a proportion of total viable cells. (G) CD4+ T cell subsets as a proportion of CD4+ T cells, corresponding to naive (Na), central memory (CM), transitional effector memory (TEM), and effector memory (EM) T cells. (H) CD20+ B cell subsets as a proportion of CD20+ B cells, corresponding to other (Other), memory (MEM), marginal zone–like (Mz-like), and naive B cells. Unpaired 2-tailed Student’s t test was used to test significance for all cell types other than mDCs, which had values of 0 in the CD group, so an unpaired 1-tailed t test was performed with Welch’s correction. *P < 0.05.