Fig. 1.
Functional LRPAP1-specific T cell exists in the repertoire of non-small cell lung adenocarcinoma patients. a PBMCs from 4 different patients were enriched for LRPAP121–30-specific CD8 T cells by peptide/HLA-A2 multimer magnetic bead isolation and stimulated with short peptide. Flow cytometry analysis was used to visualize the peptide-specific T cell frequencies with two different fluorescent labels on the multimers. b ELISPOT assay for IFNγ release by T cell bulks upon recognition of the LRPAP1 peptide. Pictures of the ELISPOT plate are depicted in Supplementary Figure 1. c, d Relative gene expression of TAP1 (c) and LRPAP1 (d) of a panel of 3 human cancer lines (wild type versus TAP-KO), and 3 non-transformed cells. e LRPAP121–30-specific polyclonal T cell culture of patient × 23 was sorted in high- and low-affinity T cells on basis of peptide/HLA-A2 multimer staining intensity and tested against the human cell panel. Cytokine concentrations were measured by IFNy ELISA