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. 2021 Dec 5;13(2):102–119. doi: 10.1007/s13238-021-00890-3

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© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Prolonged SF/a2i/L culture increases the developmental potential of AG-haESCs by ICAHCI. (A) Birth rate of the SC mice generated from TSa2i/L-derived cells with early and late passages. Passage 30 is a cutoff used to divide cells into early and late groups. SC pups obtained by cesarean section (C-section) were not used to calculate the birth rate. All error bars indicate the average mean ± SEM. P value was calculated by t test. *, P < 0.05. (B) DNA methylation levels at H19 and IG DMRs of early- and late-passage AG-haESCs derived by TSa2i/L conditions. 2i/L (2i-14) AG-haESCs are as controls. (C) Boxplot showing the DNA methylation levels of genome and gamete DMRs in 2i/L (2i-14 and 2i-O48), a2i/L (a2i-8), and TSa2i/L-derived (TSa2i-C57 and TSa2i-14) AG-ESCs. O48 is a cell line with both H19- and IG-DMR deletions derived previously (Zhong et al., 2015). N.D., not detected. (D) Snapshots of DNA methylation levels of a paternal DMR (IG-DMR) and a maternal DMR (Nespas-DMR) in 2i/L (2i-14 and 2i-O48), a2i/L (a2i-8), and TSa2i/L-derived (TSa2i-C57 and TSa2i-14) AG-ESCs. The red dashed box represents the deleted region in 2i-O48