Table 1.
Summary of SC mice derived from TSa2i/L-derived AG-haESCs
| AG-haESC line | Genetic background/gene-modifications | Passage number | No. of transferred 2-cell embryos | No. of growth-retarded pups (% transferred embryos)a | No. of normal pups (% transferred embryos)a |
|---|---|---|---|---|---|
| TSa2i-14 | C57BL/6/actin-EGFP | p23-p28 | 252 | ND | 36 (14.3) |
| p33-p54 | 460 | ND | 107 (23.3) | ||
| p60 | 144 | 2 (1.4)a | 45 (31.3)a | ||
| TSa2i-C57 | C57BL/6 | p12-p24 | 252 | ND | 43 (17.1) |
| p21 | 114 | 1 (0.9)a | 17 (14.9)a | ||
| p30-p62 | 507 | ND | 141 (27.8) | ||
| TSa2i-F1 | B6D2F1 | p12-p27 | 404 | ND | 87 (21.5) |
| p35 | 190 | 0a | 46 (24.2)a | ||
| Subtotal | p12-p30 | 1,022 | 183 (17.9) | ||
| p30-p62 | 1,301 | 339 (26.1) | |||
| TSa2i-14-Tet3 KO |
C57BL/6/actin-EGFP /Tet3 KO |
p38 | 144 | ND | 38 (26.4) |
| TSa2i-14-Tet TKO | C57BL/6/actin-EGFP/Tet1, 2, 3 knockout | p45-p47 | 243 | ND | 77 (31.7) |
| TSa2i-14-Dusp9-HA-138 | C57BL/6/actin-EGFP/Dusp9-HA knockin | p37 | 114 | ND | 37 (32.5) |
| TSa2i-14-Dusp9-HA-120 | C57BL/6/actin-EGFP/Dusp9-HA knockin | p37 | 144 | ND | 12 (8.3) |
| TSa2i-14-H19KO (H19KO-40) | C57BL/6/actin-EGFP/H19-DMR knockout | p68 & p69 | 360 | 0a | 90 (25)a |
| TSa2i-14-DKO (DKO-20) | C57BL/6/actin-EGFP/H19-DMR & IG-DMR knockout | p68 | 180 | 0a | 43 (23.9)a |
| TSa2i-C57-DKO (DKO-187 p68) | C57BL/6/H19-DMR & IG-DMR knockout | p68 | 144 | 3 (2.1)a | 32 (22.2)a |
aDerived by C-section at E18.5
ND: not detected