Fig. 3.

GmSHR4/5 function in cortical tissues during the nodule initiation and affect the formation of nodule vascular tissues. (A) Knockdown of GmSHR4 did not affect the nodule formation. (B and C) Knockdown of GmSHR5 leads to the disrupted cortical division in ground tissues. (D–I) Knockdown of both GmSHR4 and -5 leads to the disrupted cortical division in ground tissues. (J–L) Knockdown of both GmSHR4 and -5 delayed the formation of the vascular tissues in the nodule primordia. Red arrows and white asterisks point to the division of the endodermal cells and pericycle cells, respectively. Yellow dashed lines define the boundary of the division in outer ground tissues or inner ground tissues. Yellow asterisk points to the outer ground tissues. Red asterisks indicate the vascular tissues. Yellow arrows indicate the division of the outer cortical cells (n ≥ 20). n represents the number of independent biological samples. These experiments were repeated three times with similar results. (Scale bars, 100 μm.)