Fig. 1.

Study design. (A) Schematic of the reporter cell lines created by CRISPR genome editing. Either GFP or NanoLuc gene is inserted under the control of endogenous hTERT promoter in exon 1 with in-frame fusion. GFP/NanoLuc insertion and promoter mutation corrections were done using a single homologous recombination (HR) template designed to span homology arms in hTERT promoter and downstream of exon 1. (B) Workflow of the genome-wide screens. The reporter lines and the algorithms that are used to identify hits from the CRISPR and siRNA screens are indicated. Hits from siRNA and CRISPR knockout screen were overlapped to identify commonality factors and were then chosen for the validation study.