Table 3.
Comparison of major platforms incorporating CRISPR/Cas 12
| System name | Effector | Recognition type |
Sensitivity | Specificity | Signal amplification |
Quantitative | Multiplex | Time | Readout | Infrastructure requirement |
Reference |
|---|---|---|---|---|---|---|---|---|---|---|---|
| CaT-SMelor | LbCas12a | Small molecule | nM | Single molecule | N | Y | N | 40 min | Fluorescence | Substantial | [59] |
| OCTOPUS | LbCas12a | dsDNA | One copy | 1 nt | RPA | Y | N | 50 min | Fluorescence | Substantial | [60] |
| CORDS | LbCas12a | ASFV | aM | 1 nt | RAA | N | N | ~ 1 h | LFA | Few | [61] |
| Cas12aVDet | LbCas12a | Mycoplasma | aM | 1 nt | RPA | N | N | ~ 30 min | Naked eye | Few | [25] |
| CDetection | AaCas12b | HPV | aM | 1 nt | RPA | N | N | ~ 2.5 h | Fluorescence | Substantial | [62] |
| CRISPR-MTB | LbCas12a | TB | 98% | 1 nt | RPA | N | N | / | Fluorescence | Substantial | [63] |
| DETECTR-COVID19 | LbCas12a | SARS-CoV-2 | 95% | 1 nt | RT-LAMP | N | N | ~ 45 min | LFA | Few | [19] |
CaT-SMelor: CRISPR-Cas12a- and aTF-mediated small molecule detector. OCTOPUS: One-pot toolbox with precision and ultrasensitivity; higher sensitivity than general qPCR; LFA: Lateral flow assay; ASFV: African swine fever virus; CORDS: Cas12a-based on-site and rapid detection system; Cas12aVDet: Cas12a-based visual detection; CRISPR-MTB: Clinic test; CDetection: Cas12b-mediated DNA detection; DETECTR: CRISPR trans reporter