TABLE 2.
Nucleotide mismatches at primer and probe binding sites for the LCx HIV assay and the MONITOR v1.5 test
| Assay or test | Primer or probe | Mean (range) no. of nucleotide mismatches for:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Group M subtype
|
Group O | ||||||||
| A | B | C | D | CRF-AE | F | G | |||
| LCx HIV | HIV-1 forward | 0 | 0.1 (0–1) | 1.0 (0–2) | 0 | 1 (1) | 1 (1) | 1 (1) | 4 (4) |
| HIV-1 probe | 0 | 0 | 0.2 (0–1) | 0 | 0.1 (0–1) | 0 | 0 | 0 | |
| HIV-1 reverse | 1 (0–3) | 0.4 (0–2) | 0.2 (0–1) | 0 | 0 | 1 (1) | 0 | 1 (1) | |
| Total | 1 (0–3) | 0.6 (0–2) | 1.3 (0–3) | 0 | 1.1 (1–2) | 2 (2) | 1 (1) | 5 (5) | |
| MONITOR v1.5 | SK145 forward | 3.3 (3–4) | 0 | 0 | 0 | 3 (3) | 0.5 (0–1) | 2 (2) | 9.5 (9–10) |
| SK102 probe | 2 (2) | 0.4 (0–1) | 2.2 (2–3) | 0.2 (0–1) | 0.9 (0–1) | 2.5 (2–3) | 4 (4) | 7.5 (7–8) | |
| SKCC1B reverse | 0.7 (0–1) | 0.3 (0–1) | 0.3 (0–1) | 1.2 (1–2) | 0 | 2 (2) | 2 (2) | 5 (5) | |
| Total | 6 (6) | 0.7 (0–1) | 2.5 (2–4) | 1.5 (1–2) | 3.9 (3–4) | 5 (4–6) | 8 (8) | 22 (21–23) | |