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. 2021 Oct 25;102(2):143–153. doi: 10.1038/s41374-021-00682-7

Fig. 2. The importance of PLCG1 in IDHwt LGG and PLCG1 is associated with IDH status at the DNA and protein levels.

Fig. 2

a Enrichment plots of GSEA using the gene expression profiles of IDHwt LGG from the CGGA database indicated the top 5 functions that PLCG1 involved protein secretion, MYC targets, mitotic spindle, G2/M checkpoint, and E2F targets. b Representative photomicrographs of PLCG1 immunostaining of PLCG1-negative and PLCG1-positive samples. Scale bar = 50 µm. c The PLCG1 IHC score was correlated with the PLCG1 mRNA level (p < 0.05). d The PLCG1 IHC score was correlated with IDH status (p < 0.01). e Representative FISH image of nuclei of LGG cells displaying PLCG1 gene amplification and no alteration in PLCG1 copy number. PLCG1 and CEP2 (control) were labeled with red and green, respectively. CEP2, centrosome protein 2. f PLCG1 amplification was correlated with PLCG1 mRNA levels (p < 0.01). g PLCG1 amplification was correlated with the PLCG1 IHC score (p < 0.01).