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. 2021 Oct 25;102(2):143–153. doi: 10.1038/s41374-021-00682-7

Fig. 3. siRNA-mediated knockdown of PLCG1 affects the proliferation, migration and invasion of IDHwt LGG tumor cells.

Fig. 3

a Levels of PLCG1 mRNA in SW1088 and SW1783 cell lines transfected with gene-specific siRNAs (relative to those in cells with control siRNA). b The expression of PLCG1 protein examined in SW1088 and SW1783 cells by Western blotting. c Representative images of the BrdU proliferation assay reflecting the effect of siRNA on SW1088 and SW1783 cells. Scale bar = 30 µm. d Representative images of the migration assay reflecting the effect of siRNA on SW1088 and SW1783 cells. Scale bar = 20 µm. e Representative images of the invasion assay reflecting the effect of siRNA on SW1088 and SW1783 cells. Scale bar = 20 µm. f The statistical results of the BrdU proliferation assay. g The statistical results of the SW1088 and SW1783 migration assays. h The statistical results of the SW1088 and SW1783 invasion assays. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Data are expressed as the means ± SD.