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. 2021 Oct 25;102(2):143–153. doi: 10.1038/s41374-021-00682-7

Fig. 5. U73122 suppressed the proliferation and cell cycle progression and promoted apoptosis of IDHwt LGG cell lines in vitro.

Fig. 5

a Representative images of the BrdU proliferation assay, indicating the effect of U73122 on SW1088 and SW1783 cells. Scale bar = 30 µm. b The statistical results of the BrdU proliferation assay. c Representative graphs showing cell cycle distribution. d Statistical analysis of the cell cycle distribution (G1, G2, and S) of SW1088 (top) and SW1783 (down) cells in the control, DMSO, and U73122 groups. e Representative graphs showing the effect of U73122 on early and late apoptosis by annexin V–PI flow cytometry assays. f The statistical results of SW1088 (up) and SW1783 (down) apoptosis assays. ***p < 0.001, **p < 0.01, *p < 0.05. Data are expressed as the mean ± SD.