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. 2021 Nov 15;102(2):172–184. doi: 10.1038/s41374-021-00695-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Flow cytometry of barcode-labelled cells (G61) before seeding at P0, (ES 2). Expansions of cells into subcultures C1–C6 and formation of clonal population visible as “clouds” or “streaks” during propagation. Clonal formation in culture C3 (ES 2–ES 6), corresponding to passages P2, P3, and P5. B Confocal imaging of cultures C1-C6 at P2 and P5, showing formation of emerging clonal populations of barcoded cells (ES 4, 6). Scale bar corresponds to 50 µm.