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. 2021 Dec 17;298(2):101501. doi: 10.1016/j.jbc.2021.101501

Figure 5.

Figure 5

L-2HGDH expression is important for the modulation of HIF-1a in macrophages.A, HEK293 MTC cells were transfected with FLAG-tagged L-2HGDH or D-2HGDH for 24 h, prior to stimulation with LPS (100 ng/ml, 24 h). HIF-1α, IL-1β, L-2HGDH, D-2HGDH, and FLAG were measured by Western blot, n = 2. BMDMs were transfected with L-2HGDH or control siRNA (50 nM) for 144 h, prior to LPS (100 ng/ml) for 24 h, and HIF-1α and IL-1β measured by Western blot, n = 4 (B). C, expressions of L2hgdh, Il1b, Phd3, Nos2, Ldha, and D2hgdh are shown as fold over the unstimulated control, n = 3. Graphs represent the mean ± S.E.M.; ∗ p < 0.05, ∗∗ p < 0.01; one-way ANOVA. 2HGDH, 2-hydroxyglutarate dehydrogenase; BMDM, bone-marrow-derived macrophage; LPS, lipopolysaccharide.