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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: ACS Appl Mater Interfaces. 2021 Sep 2;13(36):42533–42542. doi: 10.1021/acsami.1c14285

Figure 2.

Figure 2.

(A) Schematic illustration of the bioreductive release of MEL from pacMELClv and degradation of the MEL component by α-chymotrypsin (Protein Data Bank ID 2CHA). (B) Agarose gel electrophoresis (2%) of pacMELClv treated with 10 mM DTT with varying treatment times. (C) The release profile for pacMELClv was determined by gel band densitometry analysis. (D) Polyacrylamide gel electrophoresis showing α-chymotrypsin degradation of pacMELClv, YPEG-MEL, and free MEL. The percentage of MEL degradation after 120 min of treatment is shown in (E). **P < 0.01, and ****P < 0.0001 (two-tailed t-test).