Laemmli loading buffer 2×

Reagent	Stock	Final concentration	Amount (mL)
SDS	10%	4%	200 mL
Glycerol	100%	20%	100 mL
βGP	1%	0.004%	2 mL
Tris-HCl pH 6.8	1 M	125 mM	62.5 mL
2-mercaptoethanol	n/a	1/20	25 mL
ddH2O	n/a	n/a	110.5 mL
Total	n/a	n/a	500 mL

Store at 25°C. Stable for a year.