Separating gel (4× 15% gels)

Reagent	Final concentration	Amount
EDI ultrapure water		4.4 mL
30% Acrylamide/0.8% bisacrylamide	15% acrylamide/0.4% bisacrylamide	10 mL
1.5 M Tris-HCl (pH:8.8)	390 mM	5.2 mL
10% SDS	0.1%	200 μL
10% APS	0.05%	100 μL
TEMED	0.5%	100 μL
Total		20 mL

Store at 4°C