Stacking gel (4× 15% gels)

Reagent	Final concentration	Amount
EDI ultrapure water		5.86 mL
30% Acrylamide/0.8% bisacrylamide	4.02% acrylamide/0.11% bisacrylamide	1.34 mL
0.5 M Tris-HCl (pH:6.8)	130 mM	2.6 mL
10% SDS	0.1%	100 μL
10% APS	0.1%	100 μL
TEMED	0.1%	10 μL
Total		10 mL

Store at 4°C