5× sample loading buffer

Reagent	Final concentration	Amount
Tris-HCl pH:6.8	250 mM	2.5 mL
SDS	10%	1 g
Glycerol	30%	3 mL (from 100%)
Bromophenol Blue	0.25%	5 mg
BME	5%	500 μL
EDI ultrapure water		Up to 10 mL
Total		16 mL

Store at −20°C