REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, consumables and recombinant proteins, and other
Acrylamide/bisacrylamide, 30% Solution 29:1	Sigma-Aldrich	Cat#A3574
Acrylamide/bisacrylamide, 30% Solution 29:1	Sigma-Aldrich	Cat#A3574
APS	Sigma-Aldrich	Cat#A3678
TEMED	Sigma-Aldrich	Cat#T9281
Calcium Chloride	Sigma-Aldrich	Cat#C4901
Acetic Acid	ISOLAB	Cat#901.013.1000
HCl	ISOLAB	Cat#932.106.2501
NaOH	ISOLAB	Cat#969.112.0500
SDS	ISOLAB	Cat#915.02P.0051
Beta Mercaptoethanol (BME)	Merck	Cat#8057400250
SDS-PAGE casting system	Bio-Rad	Cat#1658015
SDS-PAGE system	Bio-Rad	Cat#1658004
Nanodrop 2000c	Thermo Scientific	Cat#ND2000C
Amicon Ultra 15 mL 10K centrifugal filter unit	Amicon	Cat#UFC901024
Kimwipes	Kimberly-Clark	Cat#13258179
Innova 44R	Eppendorf, New Brunswick	Cat#16524370
Akta Go	Cytiva	Cat#29383015
Regenerated Cellulose Dialysis tubing	Thermo Scientific	Cat#68100
Tryptone	Sigma-Aldrich	T7293-1KG
Yeast Extract	Sigma-Aldrich	Y1625-1KG
IPTG	GOLDBIO	Cat#12481C100
Chloramphenicol	GOLDBIO	Cat#C-105-100
Kanamycin	GOLDBIO	Cat#K-120-100
EDI ultrapure water	Milli-Q	Cat#ZR0Q008WW
NaCl	ISOLAB	Cat#969.036.0500
Glycerol	ISOLAB	Cat#927.043.1000
Dithiothreitol (DTT)	Roche	Cat#10708984001
Tris	Sigma-Aldrich	Cat#93362
Triton X-100	Sigma-Aldrich	Cat#T9284
Ni-NTA Agarose resin	QIAGEN	Cat#30250
PreScission	GenScript	Cat#Z02799
Terasaki Plate	Greiner Bio-One	Cat#654 102
Pact Premier	Molecular Dimensions	Cat#MD1-29
Pure Cotton	Ipek	N/A
Bacterial and virus strains
Rosetta™ 2(DE3)	Sigma-Aldrich	Cat#71400-M
Deposited data
Monoclinic structure of Mpro at ambient temperature	Durdagi et al. (2021)	PDB ID:7CWB
Recombinant DNA
pET28a(+) Construct	GenScript
Software and algorithms
PSOCAKE	Damiani et al. (2016)	https://github.com/lcls-psana/psocake
CHEETAH	Barty et al. (2014)	https://github.com/biochem-fan/cheetah
CrystFEL	White et al. (2012)	https://www.desy.de/∼twhite/crystfel/
CCP4	Winn et al. (2011)	https://www.ccp4.ac.uk/
SnapGene	GSL Biotech	https://www.snapgene.com
Structure Refinement: PHENIX	Adams et al. (2010)	http://www.phenix-online.org/
Structure Modeling: COOT	Emsley and Cowtan (2004)	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Schrödinger, LLC	https://pymol.org/2/
Maestro molecular modeling program	Madhavi Sastry et al. (2013)	https://www.schrodinger.com/products/maestro
PROPKA	Bas et al. (2008)	https://pypi.org/project/propka/
R (Bio3D package)	Grant et al. (2006) and Yao and Grant (2013)	https://www.r-project.org/